Alterations of Auxin Perception in rolB-Transformed Tobacco Protoplasts (Time Course of rolB mRNA Expression and Increase in Auxin Sensitivity Reveal Multiple Control by Auxin)
نویسندگان
چکیده
منابع مشابه
Modifying the lntracellular Auxin Concentration'
Phenotypical alterations observed in rolB-transformed plants have been proposed to result from a rise in intracellular free auxin due to a RolB-catalyzed hydrolysis of auxin conjugates (J.J. Estruch, J. Schell, A. Spena [1991] EMBO J 10: 3125-3128). We have investigated this hypothesis in detail using tobacco (Nicofiana tabacum) mesophyll protoplasts isolated from plants transformed with the ro...
متن کاملRetraction Auxin inducibility and developmental expression of axi 1: a gene directing auxin independent growth in tobacco protoplasts
We (the authors) wish to report the following correction regarding our studies of deoxynucleotide-substituted U6 snRNAs in yeast spliceosomes. In our original work, we tested 50 site-specific deoxynucleotide substitutions in U6 RNA for their effects on splicing. Of these, only four specific deoxynucleotides blocked splicing and did so reproducibly. Recently, we repeated these experiments using ...
متن کاملAuxin inducibility and developmental expression of axi 1: a gene directing auxin independent growth in tobacco protoplasts.
We describe the characterization of axi 1, a tobacco gene isolated by activation T-DNA tagging which apparently plays a role in auxin action. Upon deregulated expression, axi 1 confers on protoplasts the ability to grow in culture not only in the absence of auxin but also in high auxin concentrations where maximal frequencies of cell division are not observed in wild-type protoplasts. In wild-t...
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AUXIN RESPONSE FACTOR7 (ARF7) is one of five ARF transcriptional activators in Arabidopsis thaliana that is proposed to regulate auxin-responsive expression of genes containing TGTCTC auxin response elements in their promoters. An Arabidopsis mutant (nonphototropic hypocotyl4-1 [nph4-1]) that is a null for ARF7 showed strongly reduced expression of integrated auxin-responsive reporter genes and...
متن کاملExtracellular H(2)O(2) induced by oligogalacturonides is not involved in the inhibition of the auxin-regulated rolB gene expression in tobacco leaf explants.
alpha-1,4-Linked oligogalacturonides (OGs) inhibit auxin-regulated transcriptional activation of a rolB-beta-glucuronidase (GUS) gene fusion in tobacco (Nicotiana tabacum) leaf explants (D. Bellincampi, M. Cardarelli, D. Zaghi, G. Serino, G. Salvi, C. Gatz, F. Cervone, M. M. Altamura, P. Costantino, G. De Lorenzo [1996] Plant Cell 8: 477-487). In this paper we show that inhibition by OGs is ver...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1994
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.105.4.1209